報告題目: Mechanism and versatility of Fe(II)/2-oxoglutarate hydroxylases

報 告 人: Robert P. Hausinger

Director Quantitative Biology Program, Co-Director Quantitative

Biology Initiative, Michigan State University, East Lansing, MI

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報告時間:  2012年11月12日上午10:00

報告地點:  生物藥學樓3號樓105房間

聯 系 人:  許平教授

報告人簡介：

Mechanism of enzyme action; structure-function studies of enzymes; incorporation of nickel in bacterial urease; biodegradation of sulfonate compounds; DNA repair; microbial physiology

Prof. Hausinger’s laboratory explores specific aspects of microbial physiology and enzymology related to transition metals. For example, they study mechanisms of catalysis by metalloenzymes and characterize the pathways for biosynthesis of protein metallocenters. they use an array of experimental techniques and approaches that ranges from gene cloning to enzyme kinetics, from site-directed mutagenesis to metal ion binding assays, and from active site peptide studies to biophysical spectroscopic methods.

Representative Papers

Liu, A., R. Y. N. Ho, L. Que, Jr., M J. Ryle,  B. S. Phinney, and R. P. Hausinger.  2001.  Alternative reactivity of an -ketoglutarate-dependent iron(II) oxygenase: enzyme self-hydroxylation. J. Am Chem. Soc. 123:5126-5127.

Ryle, M. J., and R. P. Hausinger.  2002. Non-heme iron oxygenases. Curr. Opin. Chem. Biol. 6:193-201.

Ryle, M. J., K. D. Koehntop, A. Liu, L. Que, Jr., and R. P. Hausinger. 2003. Interconversion of two oxidized forms of taurine/-ketoglutarate dioxygenase, a non-heme iron hydroxylase: evidence for bicarbonate binding. Proc. Natl. Acad. Sci. USA 100:3790-3795. PMC153000.

Hausinger, R. P. 2003. Ni and CO: more surprises. Nature Struct. Biol. 10:234-236.

Kuchar, J., and R. P. Hausinger. 2004. Biosynthesis of Metal Sites. Chem. Rev. 104:509-526.

Proshlyakov, D. A., T. F. Henshaw, G. R. Monterosso, M. J. Ryle, and R. P. Hausinger. 2004. Direct detection of oxygen intermediates in the non-heme Fe enzyme taurine/-ketoglutarate dioxygenase. J. Am. Chem. Soc. 126:1022-1023.

Carter, E. L., D. E. Tronrud, S. R. Taber, P. A. Karplus, and R. P. Hausinger. 2011. Iron-containing urease in a pathogenic bacterium. Proc. Natl. Acad. Sci. 108:13095-13099. PMC3156221.