報告題目：Mechanistic Studies of Polyhydroxybutyrate (PHB) Biosynthesis
報  告  人🏜：Ping Li, Assistant Professor
Department of Chemistry,
Kansas State University, Manhattan, KS, USA
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報告時間：6月10日 上午10：00
報告地點：徐匯校區哲生館112會議室
組織單位：分子微生物代謝實驗室
EON体育4平台
Postdoctoral positions and graduate assistantship are available in Dr. Li’s group.

Abstract
Mechanistic Studies of Polyhydroxybutyrate (PHB) Biosynthesis
PHB synthases catalyze the conversion of 3-hydroxybutyryl coenzyme A (HBCoA) to PHB with a molecular mass of 1.5 MDa. The polymerization involves covalent catalysis with a Cys. Two mechanisms for elongation have been proposed. The first involves an active site composed of two monomers in which the growing hydroxybutyrate (HB) chain alternates between Cys on each monomer. The second involves covalently attached intermediates and noncovalent (HB)nCoA intermediates. Three strategies have been developed to distinguish between these models. Our results support the mechanism involving covalently and noncovalently bound intermediates. In addition, a loading site is proposed for the priming process. Understanding the mechanism of polymerization will help us engineer PHB synthases to produce biodegradable polymers in a controlled fashion.