報告題目1：Stupid! That’s PLANTS!-How a Bacterium See its Vegetable

報 告  人：錢韋（Qian Wei）🥠，中科院微生物所

報告時間🫲🏽：7月6日 9:30am-10:30am

報告地點😎：閔行校區生物藥學樓2-116

聯 系  人：何亞文， 13564385795

報告摘要𓀗：To recognize the self and non-self is critical during microbial infection for both host and pathogen. To date scientists have revealed that eukaryotic cells sense the so-called pathogen-associated molecular patter (PAMP) of microbes, such as flagellin, lipopolysaccharides, peptidoglycan, and elongation factors, to trigger immune responses. However, how pathogenic bacteria detect the host environment and respond accordingly remains unclear. In recent years, we experimentally demonstrated that a plant pathogenic bacterium Xanthomonas campestris pv. campestris, the causative agent of black rot disease of cruciferous plant, employs receptor histidine kinases to directly sense various environmental cues, including quorum-sensing signal, iron depletion, and even plant hormone that modulates inter-kingdom signaling. Our study not only revealed that the host signal perceptions are of important to the bacterial pathogenesis, but also suggested that bacteria cells have intelligence quotient (IQ) with primordial thinking and judgement.

報告題目2🔀🏰：Plant microbiota from sequencing to functional study

報 告  人🙍🏿‍♂️：白洋（Yang Bai）👩‍👦，中科院遺傳與發育所

報告時間：7月6日 9:30am-10:30am

報告地點🍢😇：閔行校區生物藥學樓2-116

聯 系  人🎶：何亞文， 13564385795

報告摘要：Plants harbor abundant and diverse bacterial microbiota. Culture-independent 16S rRNA gene profiling revealed distinctive bacterial communities associated with Arabidopsis roots and leaves. The availability of comprehensive pure cultures of root- and leaf-derived bacteria is critical to deeply understand the colonization ability and organ specificity of Arabidopsis-associated bacteria in a bacterial community context. We established Arabidopsis root- and leaf-derived microbiota culture collections representing the majority of bacterial species that are reproducibly detected by previous culture-independent community sequencing. We found an extensive species intersection between the leaf and root microbiotas. Genome drafts of 400 isolates revealed a large overlap of genome-encoded functional capabilities between leaf and root-derived bacteria with few significant differences at the level of individual functional categories. Using defined bacterial communities and a gnotobiotic Arabidopsis plant system we illustrated that the isolates form bacterial communities resembling natural microbiotas on their cognate host organs, and root- and leaf-derived bacteria have specific roles in the microbiota establishment of their original niches. The comprehensive bacteria culture collections and high quality genomes together with the remarkable reproducibility of the gnotobiotic reconstitution system enable future studies on bacterial community establishment and functions under laboratory conditions (Bai et al., 2015, Nature).