Abstract👩🏻‍🚀：

Cytochrome P450 monooxygenase are a superfamily of heme enzyme with a cysteine as axial ligand, which catalyze the monooxygenation of various organic substrates under mild conditions. P450s are synthetically useful enzymes and promising versatile oxidative biocatalysts. However, the practical use of P450 is limited by its co-enzyme NAD(P)H and complexed electron transport system. Using H2O2 can simple the catalytic cycle of P450, however, most of P450s are inactive in the presence of H2O2. To overcome the problem, by mimicking the molecular structure and catalytic mechanism of natural peroxygenase and peroxidase, we have recently constructed an artificial P450 peroxygenase system with the assistance of dual-functional small molecule (DFSM) for the first time, which provides an alternative approach for the activation of the normally H2O2-inert P450BM3 enzyme. In combination with protein engineering, the DFSM-facilitated P450 peroxygenase system has been employed to the various oxidation reactions of non-native substrates, such as alkanes epoxidation, sulfoxidation of thioanisole, alkanes and aromatic hydroxylation, and so on, which showed unique activities, regio- and enantioselectivities in comparison with the natural P450 enzyme.

報告人簡介💆🏼：

叢誌奇, 博士、研究員、博士生導師💇🏻‍♂️🏊🏿‍♂️，中國科EON4青島生物能源與過程研究所👨🏻‍🦽‍➡️，單碳酶催化研究組組長。於2009年在日本熊本大學獲得理學博士學位，隨後在日本分子科學研究所和名古屋大學從事博士後研究，參與多項日本文部省基礎和重大研究課題。2016年回國工作後主持國家自然科學基金面上項目2項、青島市創新領軍人才計劃🤽、青島能源所十三五期間“一三五”重點培育方向項目等研究課題。以第一和通訊作者在J Am Chem Soc、Angew Chem Int Ed、ACS Catal等國際期刊發表研究論文20多篇🤱🏿。主要研究方向是金屬酶的化學機理解析、生物酶催化及其在合成生物技術上的應用🎫。相關研究成果被ChemistryViews、中國科學報、中科院網站、基金委網站等專題報道𓀊，並被權威學術雜誌多次正面引用和評述🙆🔱。